Rat lens aldose reductase and polyol production
نویسندگان
چکیده
منابع مشابه
The sorbitol pathway in the human lens: aldose reductase and polyol dehydrogenase.
The sorbitol pathway in human lenses is evaluated on the enzymic level. Adult lenses, normal and nondiabetic as well as diabetic cataracts, are found to contain limited levels of aldose reductase (AR) and high levels of polyol dehydrogenase (PD) relative to the animal lens. AR is confined primarily to the lens epithelium and is two to three times higher in juvenile lenses than in the adult lens...
متن کاملAldose reductase in early streptozotocin-induced diabetic rat lens.
The present study investigates the status of lens aldose reductase in the early streptozotocin-induced diabetic rat. Aldose reductase protein concentration, protein synthesis, and enzyme activity was assayed at 3 days and 14 days post-streptozotocin injection. Our results indicated that there was no significant difference between normal (control) and diabetic rat lenses in these parameters duri...
متن کاملActivators and inhibitors of lens aldose reductase.
Aldose reductase in a highly purified state is unstable. It requires the presence of thiol groups to maintain it in an active form. The enzyme apparently exists in 3 forms, only one of which is active. Tetramethylene glutaric acid (TMG) is an effective aldose reductase inhibitor. However, a relatively high level of TMG is needed to depress dulcitol synthesis in the lens incubated in a galactose...
متن کاملIsolation and Properties of Lens Aldose Reductase.
Exposure of the lens of the eye to a high concentration of an aldose, such as n-glucose, n-galactose, or n-xylose, results in the accumulation of the corresponding sugar alcohols. The high concentration which t.hese alcohols reach within the lens fibers appears to be responsible for t,he loss of clarity of the lens that is observed in experimental sugar cataracts (1). The reduced triphosphopyri...
متن کاملPenetration and binding of aldose-reductase inhibitors in the lens.
Diffusion, partitioning, and binding of two aldose-reductase inhibitors (ARI) in the rat lens were investigated under in vitro conditions. A lipophilic ARI (CT-112) and a hydrophilic ARI (AD-5467) were used as model drugs. Under lens-uptake conditions, the drug concentration in the lens increased rapidly during the initial 10 hr and reached steady state (equilibrium) after 24 hr. Despite the eq...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1987
ISSN: 0264-6021,1470-8728
DOI: 10.1042/bj2470493